Active Recombinant Human MGAT2 Protein (AA 30-447), N-6×His/GFP tagged

• Cat.No.: MGAT2-08H
• Product Overview: Recombinant Human MGAT2 Protein (AA 30-447) with N-6×His/GFP tag was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: GFP&His
• Protein Length: AA 30-447
• Description: The product of this gene is a Golgi enzyme catalyzing an essential step in the conversion of oligomannose to complex N-glycans. The enzyme has the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. Mutations in this gene may lead to carbohydrate-deficient glycoprotein syndrome, type II. The coding region of this gene is intronless. Transcript variants with a spliced 5' UTR may exist, but their biological validity has not been determined.
• Bio-activity: ≥0.6 μmol/min/mg
• Molecular Mass: ~70 kDa
• AA Sequence: RQRKNEALAPPLLDAEPARGAGGRGGDHPSVAVGIRRVSNVSAASLVPAVPQPEADNLTLRYRSLVYQLNFDQTLRNVDKAGTWAPRELVLVVQVHNRPEYLRLLLDSLRKAQGIDNVLVIFSHDFWSTEINQLIAGVNFCPVLQVFFPFSIQLYPNEFPGSDPRDCPRDLPKNAALKLGCINAEYPDSFGHYREAKFSQTKHHWWWKLHFVWERVKILRDYAGLILFLEEDHYLAPDFYHVFKKMWKLKQQECPECDVLSLGTYSASRSFYGMADKVDVKTWKSTEHNMGLALTRNAYQKLIECTDTFCTYDDYNWDWTLQYLTVSCLPKFWKVLVPQIPRIFHAGDCGMHHKKTCRPSTQSAQIESLLNNNKQYMFPETLTISEKFTVVAISPPRKNGGWGDIRDHELCKSYRRLQ
• Purity: >95%, by SDS-PAGE as visualized by Coomassie Blue Staining
• Stability: >6 months from date of receipt at -20 to -70 centigrade as supplied.
  1 month at 4 centigrade under sterile conditions after opening
• Concentration: 1 μg/μL
• Storage Buffer: Supplied as a 0.2 μm filtered solution in 20mM HEPES and 100mM NaCl buffer, pH 7.0, with 10% Glycerol.
• Preservative: 0.05 % NaN3
• Shipping: This product is shipped as 0.2μm filtered product on dry ice.

Gene Information

• Gene Name: MGAT2 alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [ Homo sapiens (human) ]
• Official Symbol: MGAT2
• Synonyms: MGAT2; alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase; GNT2; CDG2A; CDGS2; GNT-II; GLCNACTII; alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase; Beta-1,2-N-acetylglucosaminyltransferase II; N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II; UDP-N-acetylglucosamine:alpha-6-D-mannoside beta-1,2-N-acetylglucosaminyltransferase II; glcNAc-T II; mannoside acetylglucosaminyltransferase 2; mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase; EC 2.4.1.143
• Gene ID: 4247
• mRNA Refseq: NM_002408
• Protein Refseq: NP_002399
• MIM: 602616
• UniProt ID: Q10469