Active Recombinant Human MFGE8 protein, His-tagged

• Cat.No.: MFGE8-587H
• Product Overview: Recombinant Human MFGE8 protein(Q08431-1)(Met1-Cys387), fused with His tag, was expressed in Insect Cells.

Specification

• Source: Insect Cells
• Species: Human
• Tag: His
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: When 5 x 10E4 cells/well are added to Recombinant Human MFG-E8 coated plates (12.5 μg/mL, 100 μL/well), 45-85% cells will adhere after 1 hour at 37°C.
• Molecular Mass: The secreted recombinant human MFGE8 comprises 374 amino acids and has a predicted molecular mass of 42 kDa. The apparent molecular mass of rh MFGE8 is approximately 45 kDa in SDS-PAGE under reducing conditions.
• Protein length: Met1-Cys387
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 80 % as determined by SDS-PAGE
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: MFGE8 milk fat globule-EGF factor 8 protein [ Homo sapiens ]
• Official Symbol: MFGE8
• Synonyms: MFGE8; milk fat globule-EGF factor 8 protein; SPAG10, sperm associated antigen 10; lactadherin; BA46; EDIL1; hP47; HsT19888; MFG E8; OAcGD3S; SED1; sperm surface protein hP47; medin; sperm associated antigen 10; breast epithelial antigen BA46; O-acetyl disialoganglioside synthase; HMFG; MFGM; MFG-E8; SPAG10
• Gene ID: 4240
• mRNA Refseq: NM_001114614
• Protein Refseq: NP_001108086
• MIM: 602281
• UniProt ID: Q08431