Active Recombinant Human Membrane Metallo-Endopeptidase-Like 1, His-tagged

• Cat.No.: MMEL1-1981H
• Product Overview: RecombinantHuman MMEL1 protein was expressed in Murine myeloma cellline. Gly69-Trp770,with an N-terminal 6-His tag.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• ProteinLength: 69-770 a.a.
• Description: MMEL1 is azinc protease of the neprilysin (NEP) family, which also includes NEP, ECE-2,PEX, DINE, Kell and several NEPlike proteins. It is encoded by the MMEL1 genein the human genome and is also known as soluble secreted endopeptidase (SEP),Neprilysinlike 1 and Neprilysin-2. Highly expressed in testis, the cDNApredicted a type II transmembrane protein with a short cytoplasmic tail and alarge ectodomain. Both membranebound and soluble forms were comparable withregard to model substrates, pH optima and inhibitor profiles. The ectodomainof human MMEL1 was expressedwith an N-terminal His tag and purified.
• Form: Supplied as a 0.2 μmfiltered solution in Tris, NaCl and ZnCl2.
• N-terminalSequence: His
• MolecularWeight: 82 kDa
• Activity: Measuredby its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH.The specific activity is > 250 pmoles/min/μg, as measured under thedescribed conditions.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 99 kDa,reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use amanual defrost freezer and avoid repeated freezethaw cycles. 6 monthsfrom date of receipt, -20 to -70°C as supplied. 3 months,-20to-70°C under sterile conditions after opening.

Gene Information

• Gene Name: MMEL1 membranemetallo-endopeptidase-like 1 [ Homo sapiens ]
• Official Symbol: MMEL1
• Synonyms: MMEL1; membranemetallo-endopeptidase-like 1; NL1; NL2; SEP; NEP2; MMEL2; NEPII; NEP2 (m);neprilysin-2; neprilysin II; zinc metallopeptidase; soluble secretedendopeptidase; EC 3.4.24.11
• Gene ID: 79258
• mRNA Refseq: NM_033467
• Protein Refseq: NP_258428
• UniProt ID: Q495T6
• Chromosome Location: 1p36
• Function: metal ion binding; metalloendopeptidaseactivity; peptidase activity