Active Recombinant Human MAPKAPK2, His-tagged

• Cat.No.: MAPKAPK2-1426H
• Product Overview: Recombinant human MAPKAPK2 (46-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Specification

• Description: MAPKAPK2 (MAPKAP kinase2) is a Ser/Thr protein kinase which is regulated via direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, MAPKAPK2 is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 has been shown to be one of the substrates of MAPKAPK2, in vivo.
• Source: Sf9 Cells
• Species: Human
• Tag: His
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 254 nmol/min/mg
• Molecular Mass: ~41kDa
• Protein length: 46 aa-end
• Purity: >90%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: MAPKAPK2 mitogen-activated protein kinase-activated protein kinase 2 [ Homo sapiens ]
• Official Symbol: MAPKAPK2
• Synonyms: MAPKAPK2; mitogen-activated protein kinase-activated protein kinase 2; MAP kinase-activated protein kinase 2; MAPKAP kinase 2; MAPK-activated protein kinase 2; MK2; MK-2; MAPKAP-K2
• Gene ID: 9261
• mRNA Refseq: NM_004759
• Protein Refseq: NP_004750
• MIM: 602006
• UniProt ID: P49137
• Chromosome Location: 1q32
• Pathway: Activated TLR4 signalling, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; CREB phosphorylation, organism-specific biosystem; Destabilization of mRNA by Butyrate Response Factor 1 (BRF1), organism-specific biosystem; Destabilization of mRNA by Tristetraprolin (TTP), organism-specific biosystem; FAS pathway and Stress induction of HSP regulation, organism-specific biosystem; Gene Expression, organism-specific biosystem
• Function: ATP binding; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; protein serine/threonine kinase activity; signal transducer activity