Active Recombinant Human Lymphocyte-Activation Gene 3, Fc-tagged

• Cat.No.: LAG3-5675H
• Product Overview: Recombinant Human LAG3protein was expressed in Murine myeloma cell line with the Fc part of humanIgG1 Pro100-Lys330 (C-terminus) and Leu23-Leu450 (N-terminus).

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 23-450 a.a.
• Description: LAG3, also known asCD223, is a member of the immunoglobulin superfamily. The mature LAG3 proteinis a 496 amino acid (aa) membrane protein with a 421 aa extracellular regionwhich contains four IgSF domains, a 21 aa transmembrane region and a 54 aacytoplasmic region. LAG3 and CD4 molecules share < 20% aa sequencehomology but have a similar structure. Both molecules bind to MHC class II.LAG3 binds to MHC class II with higher affinity compared to CD4. Both LAG3 andCD4 genes are located on the distal part of the short arm of chromosome 12.
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Leu23
• Molecular Weight: 72.7 kDa
• Activity: Measuredby its ability to stimulate TNFα secretion by human monocytes. The ED50for this effect is typically 0.8-2.4 μg/mL.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 90 kDa,reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100μg/mL in sterile PBS
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.

Gene Information

• Gene Name: LAG3 lymphocyte-activation gene3 [ Homo sapiens ]
• Official Symbol: LAG3
• Synonyms: LAG3; lymphocyte-activationgene 3; CD223; lymphocyte activation gene 3 protein; Protein FDC; FDC;OTTHUMP00000240397; LAG-3
• Gene ID: 3902
• mRNA Refseq: NM_002286
• Protein Refseq: NP_002277
• MIM: 153337
• UniProt ID: P18627
• Chromosome Location: 12p13.3
• Function: MHC class II proteinbinding; antigen binding; transmembrane signaling receptor activity