Active Recombinant Human Ly9 protein, His-tagged

• Cat.No.: CD229-323H
• Product Overview: Recombinant Human Ly9 protein(Lys 48 - Lys 454), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 48-454 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human CD229, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD229, His,Avitag with a linear range of 0.039-2.5 μg/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 46.7 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Lys48-Lys454
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: LY9 lymphocyte antigen 9 [ Homo sapiens ]
• Official Symbol: Ly9
• Synonyms: LY9; lymphocyte antigen 9; T-lymphocyte surface antigen Ly-9; CD229; hly9; mLY9; SLAMF3; cell surface molecule Ly-9; cell-surface molecule Ly-9
• Gene ID: 4063
• mRNA Refseq: NM_001033667
• Protein Refseq: NP_001028839
• MIM: 600684
• UniProt ID: Q9HBG7