Active Recombinant Human Leucine Rich Repeat Containing 4, His-tagged

• Cat.No.: LRRC4-5680H
• Product Overview: Recombinant Human LRRC4protein was expressed in CHO with a C-terminal 6-His tag. Ala39-Lys527.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: 39-527 a.a.
• Description: LRRC4, also called NGL2or NAG14 is a 55 kDa (predicted) type I transmembrane protein that is amember of the NGL family of synaptic LRR adhesion molecules. Human LRRC4 cDNAencodes 653 amino acids (aa) that include a 38 aa signal sequence, a 489 aaextracellular domain (ECD), a 21 aa transmembrane domain, and a 105 aacytoplasmic domain. The ECD contains nine LRRs (aa 74-288), a C2 type Ig likedomain (aa 354-440), and a Thr-rich segment (aa 455-526).
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Ala39
• Molecular Weight: 55.6 kDa
• Activity: Measuredby its binding ability in a functional ELISA. When rmNetrinG2a/Fc is coatedat 5 μg/mL, rhLRRC4 binds with an apparent KD < 10 nM.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 90-110kDa, reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 400μg/mL in PBS
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.

Gene Information

• Gene Name: LRRC4 leucine rich repeatcontaining 4 [ Homo sapiens ]
• Official Symbol: LRRC4
• Synonyms: LRRC4; leucine richrepeat containing 4; NAG14; NGL-2; MGC133342; MGC133343; leucine-richrepeat-containing protein 4; netrin-G2 ligand; leucine-rich repeat-containing4; brain tumor-associated protein BAG; brain tumor associated protein LRRC4;nasopharyngeal carcinoma-associated gene 14 protein; UNQ554/PRO1111; BAG;OTTHUMP00000212281; OTTHUMP00000212284
• Gene ID: 64101
• mRNA Refseq: NM_022143
• Protein Refseq: NP_071426
• MIM: 610486
• UniProt ID: Q9HBW1
• Chromosome Location: 7q31