Active Recombinant Human Leucine Rich Repeat And Fibronectin Type III Domain Containing 3, His-tagged

• Cat.No.: LRFN3-486H
• Product Overview: Recombinanthuman LRFN3 protein with a C-terminal 6-His tag wasexpressed in Murine myeloma cell line, NS0-derived. Ser17 - Met539.

Specification

• Cat. No.: LRFN3-486H
• Description: LRFN3 is anapproximately 90 kDa member of the Lrfn family of type I transmembraneglycoproteins. Human LRFN3 is synthesized as a 628 amino acid (aa) precursorthat contains a 16 aa signal sequence, a 523 aa extracellular domain (ECD), a21 aa transmembrane region, and a 68 aa cytoplasmic region. The ECD consistsof six leucinerich repeats (LRR), an IgC2like domain, and a fibronectintypeIII domain, tandemly aligned in that order.
• Source: Mammalian cells
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Ser17
• MolecularWeight: 56.3 kDa
• Activity: Measured by theability of the immobilized protein to support the adhesion of Neuro2a mouseneuroblastoma cells (ATCC: CCL131). When 5 x 104 cells/well are added torhSALM4 coated plates (2.5 μg/mL, 100 μL/well), approximately 50% 70% willadhere after 30 minutes at 37 °C. Optimal dilutions should be determined byeach laboratory for each application.
• Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
• Purity: >90%, by SDSPAGE underreducing conditions and visualized by silver stain.
• Reconstitution: Reconstitute at 200μg/mL in PBS.
• Storage: Use amanual defrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution. 3months, -20 to -70°C under sterile conditions after reconstitution.
• Protein length: 17-539 a.a.

Gene Information

• Gene Name: LRFN3 leucine rich repeat andfibronectin type III domain containing 3 [ Homo sapiens ]
• Official Symbol: LRFN3
• Synonyms: LRFN3; leucinerich repeat and fibronectin type III domain containing 3; SALM4; FIGLER1; leucine-richrepeat and fibronectin type-III domain-containing protein 3; synapticadhesion-like molecule 4; fibronectin type III, immunoglobulin and leucinerich repeat domains 1
• Gene ID: 79414
• mRNA Refseq: NM_024509
• Protein Refseq: NP_078785
• MIM: 612809
• UniProt ID: Q9BTN0
• Chromosome Location: 19q13.13