Active Recombinant Human LAG3 protein(Met1-Leu450), mFc-tagged

• Cat.No.: LAG3-15H
• Product Overview: Recombinant Human LAG3 (NP_002277.4) (Met1-Leu450) was expressed in HEK293 with the Fc region of Mouse IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: mFc
• Protein Length: Met1-Leu450
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized LAG3 Protein, Human, Recombinant (ECD, mFc Tag) at 2 μg/mL (100 μL/well) can bind Anti-LAG3(BMS)-IgG4 Antibody (Relatlimab), the EC50 is 8-40 ng/mL.
• Molecular Mass: The recombinant human LAG3 consists of 662 amino acids and predicts a molecular mass of 72.6 kDa. As a result of glycosylation, it migRates as an approximately 87.3 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: LAG3 lymphocyte-activation gene 3 [ Homo sapiens ]
• Official Symbol: LAG3
• Synonyms: LAG3; lymphocyte-activation gene 3; lymphocyte activation gene 3 protein; CD223
• Gene ID: 3902
• mRNA Refseq: NM_002286
• Protein Refseq: NP_002277
• MIM: 153337
• UniProt ID: P18627