Active Recombinant Human L1CAM Protein, Fc-tagged, Alexa Fluor 647 conjugated

• Cat.No.: L1CAM-485HAF647
• Product Overview: Alexa Fluor 647 conjugated recombinant human L1CAM (Accession # CAA42508) was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: Fc
• Form: Lyophilized
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of Neuro-2A mouse neuroblastoma cells. When 5 × 10^4 cells/well are added to recombinant Human L1CAM Fc Chimera coated plates (25 μg/mL with 100 μL/well), > 30% will adhere for 1 hour incubation at 37 centigrade. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human L1CAM, Fc Chimera has a calculated MW of 150.4 kDa (monomer) & 55.9 kDa (truncated). In SDS-PAGE migrates as 210-225 kDa, reducing conditions.
• N-terminal Sequence Analysis: Ile 20 & Arg 864
• Purity: > 90 % by SDS-PAGE and analyzed by silver stain
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 647 via amines
  Excitation = 650 nm
  Emission = 668 nm
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.

Gene Information

• Gene Name: L1CAM L1 cell adhesion molecule [ Homo sapiens ]
• Official Symbol: L1CAM
• Synonyms: L1CAM; L1 cell adhesion molecule; antigen identified by monoclonal R1 , HSAS, HSAS1, MASA, MIC5, S10, SPG1; neural cell adhesion molecule L1; CD171; antigen identified by monoclonal R1; S10; HSAS; MASA; MIC5; SPG1; CAML1; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1
• Gene ID: 3897
• mRNA Refseq: NM_000425
• Protein Refseq: NP_000416
• MIM: 308840
• UniProt ID: P32004