Active Recombinant Human KDR protein, His-tagged

• Cat.No.: KDR-0824H
• Product Overview: Recombinant Human KDR protein(Ala20-Glu764), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 20-764 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Human VEGF165, premium grade at 2 μg/mL (100 μL/well) can bind Human VEGF R2, His Tag with a linear range of 10-156 ng/mL. Immobilized Human VEGF R2, His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human VEGF165, His,Avitag with a linear range of 2-39 ng/mL. Immobilized Human VEGF R2, His Tag at 2 μg/mL (100 μL/well), can bind pre-mixed increasing concentrations of Bevacizumab and 0.05 μg/mL (100 μL/well) Biotinylated Human VEGF165, His,Avitag with a half maximal inhibitory concentration (IC50) of 0.74 μg/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 85.2 kDa. The protein migrates as 105-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ala20-Glu764
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: KDR kinase insert domain receptor (a type III receptor tyrosine kinase) [ Homo sapiens ]
• Official Symbol: KDR
• Synonyms: KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); vascular endothelial growth factor receptor 2; CD309; FLK1; VEGFR; VEGFR2; soluble VEGFR2; fetal liver kinase 1; fetal liver kinase-1; protein-tyrosine kinase receptor Flk-1; tyrosine kinase growth factor receptor
• Gene ID: 3791
• mRNA Refseq: NM_002253
• Protein Refseq: NP_002244
• MIM: 191306
• UniProt ID: P35968