Active Recombinant Human KDR Protein, Fc/His-tagged, Alexa Fluor 555 conjugated

• Cat.No.: KDR-1697HAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant human VEGFR2 encoding amino acid residues 1 - 764 of the extracellular domain of human VEGFR2 was fused to a 6X His-tagged Fc of human IgG1 via the peptide (IEGRMD). It was expressed inNSO Cells.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: Fc&His
• Description: Vascular endothelial growth factor receptor 2 (VEGF R2) is a transmembrane glycoprotein that is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration, and differentiation.
• Form: Lyophilized
• Bio-activity: The biological activity of Human KDR was determined by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells. The expected ED50 for this effect is typically 4-8 ng/mL in the presence of 5 ng/mL recombinant human VEGF165.
• Molecular Mass: 160-170 kDa
• Endotoxin: < 1.0 EU/ μg as determined by the LAL method
• Purity: > 90 % by SDS-PAGE and analyzed by silver stain
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: This lyophilized protein is stable for 6 to 12 months when stored desiccated at -20 to -70 centigrade. After aseptic reconstitution, this protein may be stored at 2 to 8 centigrade for one month or at -20 to -70 centigrade in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
• Storage: This lyophilized protein is stable for six to twelve months when stored desiccated at -20 to -70 centigrade. After aseptic reconstitution, this protein may be stored at 2 to 8 centigrade for one month or at -20 to -70 centigrade in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Gene Information

• Gene Name: KDR kinase insert domain receptor (a type III receptor tyrosine kinase) [ Homo sapiens ]
• Official Symbol: KDR
• Synonyms: KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); FLK1; CD309; VEGFR; VEGFR2; Vascular endothelial growth factor receptor 2; VEGFR-2; Kinase insert domain receptor; Protein-tyrosine kinase receptor Flk-1; EC 2.7.10.1; fetal liver kinase-1; soluble VEGFR2; tyrosine kinase growth factor receptor
• Gene ID: 3791
• mRNA Refseq: NM_002253
• Protein Refseq: NP_002244
• MIM: 191306
• UniProt ID: P35968