Active Recombinant Human ITGA1 Protein, C-6×His-tagged

• Cat.No.: ITGA1-27H
• Product Overview: Recombinant Human Integrin alpha 1 (Met1-Pro1141, Acidic Tail) with a 6×His tag at C-terminus and Human Integrin beta 1 (Gln21-Asp728, Basic Tail) was expressed in Chinese Hamster Ovary cell line.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: Integrin alpha 1: Met1-Pro1141
  Integrin beta 1: Gln21-Asp728
• Description: This gene encodes the alpha 1 subunit of integrin receptors. This protein heterodimerizes with the beta 1 subunit to form a cell-surface receptor for collagen and laminin. The heterodimeric receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. The alpha 1 subunit contains an inserted (I) von Willebrand factor type I domain which is thought to be involved in collagen binding.
• Bio-activity: Measured by its binding ability in a functional ELISA. When Collagen I is immobilized at 5 μg/mL, Recombinant Human Integrin alpha 1 beta 1 can bind with an apparent KD <5 nM.
  Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Predicted Molecular Mass: 132.3 kDa (Integrin alpha 1) & 86.4 kDa (Integrin beta 1)
  SDS-PAGE: 180-200 & 110-130 kDa, reducing conditions
• N-terminal Sequence Analysis: Phe29 (Integrin alpha 1) & No results obtained: Gln21 predicted (Integrin beta 1), sequencing might be blocked
• Endotoxin: <1.0 EU/μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: ITGA1 integrin subunit alpha 1 [ Homo sapiens (human) ]
• Official Symbol: ITGA1
• Synonyms: ITGA1; integrin subunit alpha 1; VLA1; CD49a; integrin alpha-1; CD49 antigen-like family member A; VLA-1; laminin and collagen receptor; very late activation protein 1; very late antigen-1
• Gene ID: 3672
• mRNA Refseq: NM_181501
• Protein Refseq: NP_852478
• MIM: 192968
• UniProt ID: P56199