Active Recombinant Human ISM1 Protein (Ser27-Tyr464), C-6×His-tagged

• Cat.No.: ISM1-33H
• Product Overview: Recombinant Human ISM1 Protein (Ser27-Tyr464) with a C-terminal 6-His tag was expressed in Chinese Hamster Ovary cell line.

Specification

• Species: Human
• Source: CHO
• Tag: His
• ProteinLength: Ser27-Tyr464
• Description: Acts as an angiogenesis inhibitor. Predicted to be involved in negative regulation of angiogenesis. Predicted to be located in extracellular region.
• Bio-activity: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human Isthmin 1/ISM1, immobilized at 5 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.
• Molecular Mass: Predicted Molecular Mass: 50 kDa
  SDS-PAGE: 62-71 kDa, reducing conditions
• N-terminal Sequence Analysis: Ser27
• Endotoxin: <0.10 EU/μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• Storage: 12 months from date of receipt, ≤ -20 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: ISM1 isthmin 1 [ Homo sapiens (human) ]
• Official Symbol: ISM1
• Synonyms: ISM1; isthmin 1; ISM; Isthmin; C20orf82; bA149I18.1; dJ1077I2.1; isthmin-1; isthmin 1 homolog; isthmin 1, angiogenesis inhibitor
• Gene ID: 140862
• mRNA Refseq: NM_080826
• Protein Refseq: NP_543016
• MIM: 615793
• UniProt ID: B1AKI9