Active Recombinant Human Integrin Alpha 5 Beta 3 Heterodimer Protein

• Cat.No.: ITGAVB3-101H
• Product Overview: Recombinant human Integrin alpha 5 beta 3 heterodimer protein (Human ITGA5 & ITGB3) Phe 42 - Tyr 995 (Integrin alpha 5, NP_002196) and Gly 27 - Asp 718 (Integrin beta 3, AAI27667) was co-expressed in human 293 cells (HEK293).

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Protein Length: 42-995;27-718 a.a.
• Description: The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
• Molecular Mass: Subunit Integrin alpha 5 (ITGA5) is fused with Strep II-tag at the C-terminus and subunit Integrin beta 3 (ITGB3) is fused with his-tag at the C-terminus. The heterodimer Human ITGA5 & ITGB3, co-expressed by ITGA5 plasmid and ITGB3 plasmid, has a calculated MW of 105.4 kDa ( alpha 5 subunit) and 77.3 kDa (beta 3 subunit). The predicted N-terminus is Phe42 (Integrin alpha 5) and Gly 27 (Integrin beta 3) . DTT-reduced Protein migrates as 105-150 kDa due to glycosylation.
• Formulation: Lyophilized from 0.22 μm filtered solution in 20 mM Tris, pH 7.5 with 150 mM NaCl and 0.5 mM CaCl2. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Purity: >90% as determined by SDS-PAGE.
• Bioactivity: Measured by its ability in functional ELISA. Immobilized recombinant human Vitronectin protein at 1 ug/ml can bind Recombinant human Integrin alpha 5 beta 3 heterodimer protein with apparent KD <8 nM.
• Endotoxin: Less than 1.0 EU per μg of the Human ITGA5 & ITGB3 by the LAL method.
• Storage: -20°C to -70°C for 3 months under sterile conditions after reconstitution. Avoid repeated freeze­thaw cycles.
• OfficialSymbol: ITGA5

Gene Information

• Gene Name: ITGA5 integrin, alpha 5 (fibronectin receptor, alpha polypeptide) [ Homo sapiens ]
• Synonyms: ITGA5; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); FNRA; CD49e; VLA5A; integrin alpha-5; VLA-5; integrin alpha-F; CD49 antigen-like family member E; fibronectin receptor subunit alpha; fibronectin receptor, alpha subunit; very late activation protein 5, alpha subunit; Integrin alpha-5; Fibronectin receptor subunit alpha; Integrin alpha-F
• Gene ID: 3678
• mRNA Refseq: NM_002205
• Protein Refseq: NP_002196
• MIM: 135620
• UniProt ID: P08648
• Chromosome Location: 12q11-q13
• Pathway: Angiopoietin receptor Tie2-mediated signaling; Arrhythmogenic right ventricular cardiomyopathy (ARVC); Axon guidance; Bacterial invasion of epithelial cells; Cell surface interactions at the vascular wall; Dilated cardiomyopathy; ECM-receptor interaction; Focal Adhesion; Focal adhesion; Hematopoietic cell lineage; Hemostasis; Integrin cell surface interactions; Integrin-mediated cell adhesion; L1CAM interactions; Pertussis
• Function: protein binding; metal ion binding; platelet-derived growth factor receptor binding; vascular endothelial growth factor receptor 2 binding