Active Recombinant Human Insulin-Like Growth Factor Binding Protein 1

• Cat.No.: IGFBP1-5048H
• Product Overview: Recombinant Human IGFBP1 was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: Human
• Tag: Non
• Description: IGF-BPs controls the distribution, function and activity of IGFs in various cell tissues and body fluids. Currently there are seven named IGF-BPs that form high affinity complexes with both IGF-I and IGF-II. IGF-BP1 is a 25.4 kDa cysteine-rich secreted protein expressed in liver, deciduas, and kidneys and is the most abundant IGF-BP in amniotic fluid. Levels of IGF-BP1 in serum are lowest after food. IGF-BP1 binds to both IGF-I and IGF-II with equal affinity.
• Form: Supplied as a lyophilized powder.
• Purity: >97% pure by SDS PAGE and HPLC
• Sequence: Region of IGF BP1 protein corresponding to amino acids MAPWQCAPCS AEKLALCPPV SASCSEVTRS AGCGCCPMCA LPLGAACGVA TARCARGLSC RALPGEQQPL HALTRGQGAC VQESDASAPH AAEAGSPESP ESTEITEEEL LDNFHLMAPS EEDHSILWDA ISTYDGSKAL HVTNIKKWKE PCRIELYRVV ESLAKAQETS GEEISKFYLP NC
• Molecular Mass: 25.4 kDa
• Applications: Numerous applications are possible with this product and should be tested by the end user under their own laboratory conditions
• Endotoxin Levels: Endotoxin level is less than 0.1 ng per ug (1 EU/ug).
• Bioactivity: The ED50 was determined by its ability to inhibit IGF-I induced proliferation of MCF-7 is 0.5 ug/ml in the presence of 6 ng/ml of human IGF-I.
• Storage: Numerous applications are possible with this product and should be tested by the end user under their own laboratory conditions

Gene Information

• Gene Name: IGFBP1 insulin-like growth factor binding protein 1 [ Homo sapiens ]
• Official Symbol: IGFBP1
• Synonyms: insulin-like AFBP; PP12; IGF-BP25; hIGFBP-1; IGFBP1; IGFBP-1; IBP-1; IGF-binding protein 1; insulin-like growth factor binding protein 1; binding protein-25; binding protein-26; binding protein-28; placental protein 12; IGF-binding protein 1; amniotic fluid binding protein; growth hormone independent-binding protein; alpha-pregnancy-associated endometrial globulin; OTTHUMP00000159561; OTTHUMP00000207990; OTTHUMP00000214348
• Gene ID: 3484
• mRNA Refseq: NM_000596
• Protein Refseq: NP_000587
• MIM: 146730
• UniProt ID: P08833
• Chromosome Location: 7p13-p12
• Pathway: Diabetes pathways; FOXA2 and FOXA3 transcription factor networks; HIF-1-alpha transcription factor network; Myometrial Relaxation and Contraction Pathways; PERK regulated gene expression; Regulation of Insulin-like Growth Factor (IGF) Activity by Insulin-like Growth Factor Binding Proteins (IGFBPs); Unfolded Protein Response
• Function: insulin-like growth factor binding