Active Recombinant Human IL7R protein, Fc/Avi-tagged, Biotinylated

• Cat.No.: IL7R-051H
• Product Overview: Biotinylated Recombinant Human IL7R(Glu21-Asp239) protein, fused to Fc/Avi tag at the C-terminus, was expressed in HEK293 cells .

Specification

• Description: Interleukin 7 Receptor alpha (IL7 R alpha ), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival (1, 2). Mature human IL-7 R alpha consists of a 219 amino acid (aa) extracellular domain (ECD) with one fibronectin type-III domain and a WSXWS motif, a 25 aa transmembrane segment, and a 195 aa cytoplasmic domain (3). Alternate splicing of human IL-7 R alpha generates a secreted soluble form of the receptor (3). Within the ECD, human IL‑7 R alpha shares 67% aa sequence identity with mouse and rat IL-7 R alpha. IL-7 R alpha associates with the common gamma chain ( gamma c) to form the functional high affinity IL-7 receptor complex (4). The gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. Human and mouse IL‑7 show cross-species activity through the IL-7 receptor (3, 5). IL-7 R alpha is expressed on double negative (CD4-/CD8-) and CD4+ or CD8+ single positive T cells as well as on CD8+ memory T cells and their precursors (6, 7). It is expressed early in B cell development, prior to the appearance of surface IgM (6). In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development (8). In human, by contrast, it is required for T cell but not for B cell development (9). IL-7 induces the downregulation and shedding of cell surface IL‑7 R alpha (10). IL-7 R alpha additionally associates with TSLP R to form the functional receptor for thymic stromal lymphopoietin (11, 12). TSLP indirectly regulates T cell development by modulating dendritic cell activation (2, 13). Knockout of TSLP R in mice provokes minor changes in B and T cell development compared to those seen with IL-7 R alpha deletion (8, 14). The complexity of IL-7 R alpha biology is suggested by the competition between IL-7 and TSLP for receptor binding and by the ability of IL-7 R alpha to form functional complexes with SCF R and HGF R (11, 12, 15, 16).
• Source: HEK293 cells
• Species: Human
• Tag: Fc&Avi
• Predicted N Terminal: Glu21
• Form: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA. When Recombinant Human IL7 is immobilized at 1 µg/mL (100 µL/well), it binds to Biotinylated Recombinant Human IL7R Fc Chimera Avi-tag with an ED50 of 0.1-1 μg/mL.
• Molecular Mass: 74-103 kDa, under reducing conditions
• Protein length: Glu21-Asp239
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Applications: Bioactivity
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.

Gene Information

• Gene Name: IL7R interleukin 7 receptor [ Homo sapiens ]
• Official Symbol: IL7R
• Synonyms: IL7R; interleukin 7 receptor; interleukin-7 receptor subunit alpha; CD127; IL-7RA; CD127 antigen; IL-7R subunit alpha; IL-7 receptor subunit alpha; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; ILRA; IL7RA; CDW127; IL-7R-alpha
• Gene ID: 3575
• mRNA Refseq: NM_002185
• Protein Refseq: NP_002176
• MIM: 146661
• UniProt ID: P16871