Active Recombinant Human IL23R protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: IL23R-0272H
• Product Overview: Biotinylated Recombinant Human IL23R protein(Gly24-Gly355), fused with Fc and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: 24-355 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human IL-23 R, Fc,Avitag at 5 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate can bind Human IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free with a linear range of 39-156 ng/mL.Immobilized Biotinylated Human IL-23 R, Fc,Avitag at 5 μg/mL (100 μL/well), can bind pre-mixed increasing concentrations of Human IL23A/p19 Neutralizing Antibody and 0.25 μg/mL (100 μL/well) Human IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free with a half maximal inhibitory concentration (IC50) of 0.3889 μg/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 66.3 kDa. The protein migrates as 95-105 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Gly24-Gly355
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL23R interleukin 23 receptor [ Homo sapiens ]
• Official Symbol: IL23R
• Synonyms: IL23R; interleukin 23 receptor; interleukin-23 receptor; IL 23R; IL-23 receptor
• Gene ID: 149233
• mRNA Refseq: NM_144701
• Protein Refseq: NP_653302
• MIM: 607562
• UniProt ID: Q5VWK5