Active Recombinant Human IL2 Protein (Ala21-Thr153, Cys145Ser), GMP Grade, Animal-Free

• Cat.No.: IL2-27HG
• Product Overview: GMP recombinant human IL-2 protein (Ala21-Thr153, Cys145Ser), with and without an N-terminal Met was expressed in E. coli, produced in an Animal-Free laboratory and manufactured and tested under cGMP guidelines.

Specification

• Species: Human
• Source: E.coli
• Protein Length: Ala21-Thr153, Cys145Ser
• Description: This gene is a member of the interleukin 2 (IL2) cytokine subfamily which includes IL4, IL7, IL9, IL15, IL21, erythropoietin, and thrombopoietin. The protein encoded by this gene is a secreted cytokine produced by activated CD4+ and CD8+ T lymphocytes, that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2R) is a heterotrimeric protein complex whose gamma chain is also shared by IL4 and IL7. The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
• Bio-activity: Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 for this effect is 0.0300-0.250 ng/mL.
  The specific activity of recombinant human IL-2 is >5.00 x 106 IU/mg, which is calibrated against the human IL-2 WHO International Standard.
• Molecular Mass: Predicted Molecular Mass: 15.5 kDa
  SDS-PAGE: 13 kDa, under reducing conditions.
• N-terminal Sequence Analysis: Met-Ala21-Pro-Thr-Ser-Ser-Ser-Thr-Lys-Lys & Ala21-Pro-Thr-Ser-Ser-Ser-Thr-Lys-Lys-Thr
• Endotoxin: <0.01 EU/ μg of the protein by the LAL method.
• Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  A minimum of 12 months when stored at ≤ -20 centigrade as supplied. Refer to lot specific COA for the Use by Date.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in Sodium Acetate with Trehalose.
• Reconstitution: Reconstitute at 500 μg/mL in sterile deionized water.
• Shipping: The product is shipped with polar packs.

Gene Information

• Gene Name: IL2 interleukin 2 [ Homo sapiens ]
• Official Symbol: IL2
• Synonyms: IL2; interleukin 2; interleukin-2; IL 2; T cell growth factor; TCGF; aldesleukin; involved in regulation of T-cell clonal expansion; IL-2; lymphokine
• Gene ID: 3558
• mRNA Refseq: NM_000586
• Protein Refseq: NP_000577
• MIM: 147680
• UniProt ID: P60568