Active Recombinant Human IL1B

• Cat.No.: IL1B-339H
• Product Overview: Human IL1B (P01584, 117 a.a.-269 a.a.) partial recombinant protein expressed in Escherichia coli.

Specification

• Species: Human
• Source: Human
• Tag: Non
• Description: Interleukin-1 beta (IL-1μ) also known as catabolin, is a cytokine protein that in humans is encoded by the IL1B gene. IL-1μ precursor is cleaved by caspase 1 (interleukin 1 beta convertase). Cytosolic thiol protease cleaves the product to form mature IL-1μ. IL-1μ is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis.
• Sequence: APVR SLNCTLRDSQ QKSLVMSGPY ELKALHLQGQ DMEQQVVFSM SFVQGEESND KIPVALGLKE KNLYLSCVLK DDKPTLQLES VDPKNYPKKK MEKRFVFNKI EINNKLEFES AQFPNWYIST SQAENMPVFL GGTKGGQDIT DFTMQFVSS
• Theoretical MW (kDa): 17
• Form: Lyophilized
• Preparation Method: Escherichia coli expression system
• Purity: > 97% by SDS-PAGE and HPLC
• Endotoxin Level: < 0.1 ng/ug (1 EU/ug)
• Activity: The ED50 as determined by the dose-dependent proliferation of murine D10S cells, was found to be μ2.0 pg/ml, corresponding to a specific activity of μ2 x 109 units/mg.
• Application: SDS-PAGE
• Storage Buffer: Lyophilized from PBS, pH 7.0
• Storage: Store at -20°C on dry atmosphere for 2 years. After reconstitution with deionized water, store at 4°C for 1 month or store at -20°C for 6 months. Aliquot to avoid repeated freezing and thawing.
• Unitprot ID: P01584
• Pathways: Alzheimer"s disease; Apoptosis; Cytokine-cytokine receptor interaction; Hematopoietic cell lineage; MAPK signaling pathway; Toll-like receptor signaling pathway; Type I diabetes mellitus.

Gene Information

• Gene Name: IL1B interleukin 1, beta [ Homo sapiens ]
• Official Symbol: IL1B
• Synonyms: IL-1; IL1F2; IL1-BETA; Catabolin; IL-1B; pro-interleukin-1-beta; Interleukin-1 beta precursor; interleukin 1, beta; preinterleukin 1 beta; IL1B_HUMAN.
• Gene ID: 3553
• mRNA Refseq: NM_000576
• Protein Refseq: NP_000567
• MIM: 147720
• Chromosome Location: 2q14
• Function: interleukin-1 receptor binding; protein binding; signal transducer activity