Active Recombinant Human IL18

• Cat.No.: IL18-1237H
• Product Overview: Recombinant human IL-18 produced in Yeast is a single polypeptide chain containing 157 amino acids, and having a predicted molecular mass of approximately 18.0kDa.

Specification

• Species: Human
• Source: Yeast
• Tag: Non
• Protein Length: 157 a.a.
• Description: Interleukin 18 (IL-18) is a cytokine which identified as a costimulatory factor for production of interferon-γ (IFN-γ ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24-kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-γ is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-γ activates macrophages and enhances NK activity and B cell maturation, proliferation and Ig secretion. IFN-γ also induces expression of MHC class I and II antigens and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-γ . Pleiotropic effects of IL-18 have also been reported, such as, enhancement production of IFN-γ and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-γ in T cells, enhancement of Fas ligand expression by Th1 cells.
• Form: Lyophilized from a 0.2μm filtered solution in 50mM NaAc, 2mM DTT and 1% sucrose, pH5.5.
• Bio-activity: Induction of IFN-γ by PBMC cell in response to the recombinant human IL-18 was measured using human IFN-γ ELISA. IFN-γ producing activity of the sample cells can be varied depending on cell conditions. Optimal concentration for each application should be determined by each laboratory.
• Endotoxin: ≤1EU/μg, determined by the LAL method.
• Purity: ≥97%, as determined by SDS-PAGE and HPLC method.
• Usage: FOR RESEARCH ONLY
• Storage: Lyophilized samples are stable for greater than six months from date of receipt at -20 centigrade to -70 centigrade. The reconstituted samples can be stored under sterile conditions at 2-8 centigrade for one month or at -20 centigrade to -70 centigrade for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of not less than 100μg/ml of the cytokine.

Gene Information

• Gene Name: IL18 interleukin 18 [ Homo sapiens (human) ]
• Official Symbol: IL18
• Synonyms: IGIF; IL-18; IL-1g; IL1F4; interleukin-18; IFN-gamma-inducing factor; IL-1 gamma; iboctadekin; interleukin 18 (interferon-gamma-inducing factor); interleukin-1 gamma
• Gene ID: 3606
• mRNA Refseq: NM_001243211
• Protein Refseq: NP_001230140
• MIM: 600953
• UniProt ID: Q14116
• Chromosome Location: 11q22.2-q22.3
• Pathway: African trypanosomiasis, organism-specific biosystem; Cellular roles of Anthrax toxin, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem
• Function: cytokine activity; protein binding

Q&As

What are the difference between IL18-01HG, IL18-153HG and IL18-500H. (sequence, biological activity, QC, or what do you propose to buy)

IL18-01HG: Sequence: a.a.37-193 Endotoxin: <20 EU/mg Bio-activity: >5.0 x 10^4 IU/mg QC: Activity test, SDS-PAGE, LAL, SEC-HPLC IL18-153HG: Protein length: Tyr37-Asp193 AA Sequence: YFGKLESKLS VIRNLNDQVL FIDQGNRPLF EDMTDSDCRD NAPRTIFIISMYKDSQPRGM AVTISVKCEK ISTLSCENKI ISFKEMNPPD NIKDTKSDIIFFQRSVPGHD NKMQFESSSY EGYFLACEKE RDLFKLILKK EDELGDRSIMFTVQNEDVD Bio-activity: 1.0*10^6IU Measured by its ability to induce IFN-gamma secretion by KG‐1 human acute myelogenous leukemia cells in the presence of TNF-alpha. QC: Activity test, SDS-PAGE, LAL IL18-500H Recombinant Human IL18 will be custom produced upon order. All the sequence, purity, activity, and QC procedures will be optional and decided by our customer.