Active Recombinant Human IL17RA protein(Met1-Trp320), His-tagged
| Cat.No. : | IL17RA-2197H |
| Product Overview : | Recombinant Human IL17RA (NP_055154.3) (Met 1-Trp 320) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus. |
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| Source : | HEK293 |
| Species : | Human |
| Tag : | His |
| Protein length : | Met1-Trp320 |
| Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
| Bio-activity : | 1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL17A at 2 μg/ml (100 μl/well) can bind biotinylated human IL17RA with a linear range of 1.28-32 ng/ml. 2. Measured by its ability to inhibit IL-17-induced IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.2‑1.2μg/mL in the presence of 5 ng/mL recombinant human IL‑17. |
| Molecular Mass : | The recombinant human IL17RA consists of 299 amino acids and has a predicted molecular mass of 35 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh IL17RA is approximately 52-60 kDa due to glycosylation. |
| Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Purity : | > 97 % as determined by SDS-PAGE |
| Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Gene Name | IL17RA interleukin 17 receptor A [ Homo sapiens ] |
| Official Symbol | IL17RA |
| Synonyms | IL17RA; interleukin 17 receptor A; IL17R, interleukin 17 receptor; interleukin-17 receptor A; CD217; CDw217; hIL 17R; IL 17RA; IL-17 receptor A; IL17R; CANDF5; IL-17RA; hIL-17R; FLJ32274; FLJ36453; MGC10262; |
| Gene ID | 23765 |
| mRNA Refseq | NM_014339 |
| Protein Refseq | NP_055154 |
| MIM | 605461 |
| UniProt ID | Q96F46 |
Not For Human Consumption!
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