Active Recombinant Human IL13RA1 protein, Fc/Avi-tagged, Biotinylated

• Cat.No.: IL13RA1-051H
• Product Overview: Biotinylated Recombinant Human IL13RA1(Ala27-Thr343) protein, fused to Fc/Avi tag at the C-terminus, was expressed in CHO cells .

Specification

• Species: Human
• Source: CHO
• Tag: Avi&Fc
• Protein Length: Ala27-Thr343
• Description: IL13 RA1, also known as IL13R and IL13 RA, is a type I transmembrane protein. Its cDNA encodes a 427 aa precursor protein, with 322 aa extracellular domain, 24 aa transmembrane domain and 60 aa intracellular domain. Within the extracellular domain, human IL13 RA1 shares 75% and 74% homology with mouse and rat IL13 RA1, respectively. IL13 RA1 expresses ubiquitously in all tissues with the highest level in heart, liver, skeletal muscle and ovary (1). As a receptor, IL13 RA1 can function alone or as a heterodimer with IL-4R. Although both IL-4 and IL-13 signal through IL-4R/IL-13 RA1 heterodimer, there are distinct differences. IL-4 binds IL-4R with high affinity then binds IL-13 RA1 with low affinity. In contrast, IL-13 binds IL-13 RA1 with decent affinity, then binds IL-4R with high affinity (2). In addition, the N-terminal Fibronectin type III domain (D1) of IL-13 RA1 is only required for the binding of IL-13 not IL-4 (3,4). After binding to IL-4 or IL-13, the Tyr residues in the cytoplasmic domain of IL-13 RA get phosphorylated and then activate signaling proteins including Jak1, Tyk1, Tyk2, IRS-1, and STAT6 (5, 6). Alternative splicing generates soluble iL-13 RA1 missing the transmembrane domain (7). It not only functions as a decoy receptor for IL-13, but also is able to reduce fasting blood glucose, mediated by IL-4 (8). Higher expression of IL-13 RA1 are found in several cancers, often associated with poor prognosis in patients (9-11). Our Avi-tag Biotinylated human IL-13 RA1 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.
• Predicted N Terminal: Ala27
• Form: Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA. When Biotinylated Recombinant Human IL13RA1 Fc Chimera Avi-tag is immobilized at 1 µg/mL (100 µL/well), Recombinant Human IL13 binds with an ED50 of 10-80 ng/mL.
• Molecular Mass: 93-103 kDa, under reducing conditions
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Applications: Bioactivity
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.6 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after opening.3 months, -20 to -70 °C under sterile conditions after opening.

Gene Information

• Gene Name: IL13RA1 interleukin 13 receptor, alpha 1 [ Homo sapiens ]
• Official Symbol: IL13RA1
• Synonyms: IL13RA1; interleukin 13 receptor, alpha 1; interleukin-13 receptor subunit alpha-1; CD213a1; CD213a1 antigen; IL 13Ra; IL13 receptor alpha 1 chain; NR4; CT19; IL-13RA1; IL-13R-alpha-1; IL-13R subunit alpha-1; cancer/testis antigen 19; IL13 receptor alpha-1 chain; IL-13 receptor subunit alpha-1; bB128O4.2.1 (interleukin 13 receptor, alpha 1); CD213A1; IL-13Ra
• Gene ID: 3597
• mRNA Refseq: NM_001560
• Protein Refseq: NP_001551
• MIM: 300119
• UniProt ID: P78552