Active Recombinant Human IL-23 Protein

• Cat.No.: IL23A-05H
• Product Overview: Recombinant Human IL-23 p40 (Ile23-Ser328) and Human IL-23 p19 (Arg20-Pro189) without tag was expressed in Spodoptera frugiperda, Sf 21.

Specification

• Source: Sf 21
• Species: Human
• Bio-activity: Measured by its ability to induce IL-17 secretion by mouse splenocytes. The ED50 for this effect is 0.05-0.3 ng/mL.
• Molecular Mass: Predicted Molecular Mass: 55.0 kDa (IL-23 single chain); 36.5 kDa (p40) & 18.5 kDa (p19)
  SDS-PAGE: 65 kDa, 43-45 kDa and 20 kDa, reducing conditions
• N-terminal Sequence Analysis: Ile23 (IL-23 single chain & p40) & Ala21 (p19)
• Endotoxin: <1.0 EU/μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in MES, NaCl and EDTA with BSA as a carrier protein.
• Reconstitution: Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
• Shipping: The product is shipped at ambient temperature.
• Protein length: 23-328;20-189 a.a.

Gene Information

• Gene Name: IL23A interleukin 23 subunit alpha [ Homo sapiens (human) ]
• Official Symbol: IL23A
• Synonyms: IL23A; interleukin 23 subunit alpha; P19; SGRF; IL-23; IL-23A; IL23P19; interleukin-23 subunit alpha; IL-23 subunit alpha; IL-23-A; IL-23p19; JKA3 induced upon T-cell activation; interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; interleukin-23 subunit p19; interleukin-six, G-CSF related factor
• Gene ID: 51561
• mRNA Refseq: NM_016584
• Protein Refseq: NP_057668
• MIM: 605580
• UniProt ID: Q9NPF7

Q&As

Is this bioactive cyno IL-23 heterodimer (p19/p40) or just the cyno IL-23p19 subunit?

Please inquiry our product manager with specific product.