Active Recombinant Human IgG3Fc protein

• Cat.No.: IgG3Fc-1573H
• Product Overview: Recombinant Human IgG3Fc protein(Glu99-Lys377) was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• ProteinLength: 99-377 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5.
• Bio-activity: Human FCGRT&B2M Heterodimer Protein, His Tag captured on CM5 Chip via anti-His antibody can bind Human IgG3 Fc, Tag Free with an affinity constant of 2.82 μM as determined in SPR assay (Biacore 8K).
• Molecular Mass: This protein carries no "tag".The protein has a calculated MW of 31.2 kDa. The protein migrates as 35-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Glu99-Lys377
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.