Active Recombinant Human IFNA2 Protein (24-188aa)

• Cat.No.: IFNA2-10H
• Product Overview: Interferon alpha-2b was expressed in E. coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Specification

• Species: Human
• Source: E.coli
• Protein Length: 24-188aa
• Description: This gene is a member of the alpha interferon gene cluster on chromosome 9. The encoded cytokine is a member of the type I interferon family that is produced in response to viral infection as a key part of the innate immune response with potent antiviral, antiproliferative and immunomodulatory properties. This cytokine, like other type I interferons, binds a plasma membrane receptor made of IFNAR1 and IFNAR2 that is ubiquitously expressed, and thus is able to act on virtually all body cells. The encoded protein is effective in reducing the symptoms and duration of the common cold and in treating many types of cancer, including some hematological malignancies and solid tumors. A deficiency of type I interferon in the blood is thought to be a hallmark of severe COVID-19 and may provide a rationale for a combined therapeutic approach.
• Form: Liquid
• Bio-activity: Measured by its binding ability in a functional ELISA with Human IFN-alpha/beta R2 in the presence of Human IFN-alpha/beta R1.
• Molecular Mass: 19.4 kDa (166aa) confirmed by MALDI-TOF
• AA Sequence: MCDLPQTHSLGSRRTLMLLAQMRRISLFSCLKDRHDFGFPQEEFGNQFQKAETIPVLHEMIQQIFNLFSTKDSSAAWDETLLDKFYTELYQQLNDLEACVIQGVGVTETPLMKEDSILAVRKYFQRITLYLKEKKYSPCAWEVVRAEIMRSFSLSTNLQESLRSKE
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 95% by SDS-PAGE
• Applications: SDS-PAGE, Bioactivity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/mL (determined by Bradford assay)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4), 10% glycerol

Gene Information

• Gene Name: IFNA2 interferon alpha 2 [ Homo sapiens (human) ]
• Official Symbol: IFNA2
• Synonyms: IFNA2; interferon alpha 2; IFNA; IFNA2B; leIF A; IFN-alphaA; IFN-alpha-2; interferon alpha-2; alpha-2a interferon; interferon alpha 2a; interferon alpha 2b; interferon alpha A
• Gene ID: 3440
• mRNA Refseq: NM_000605
• Protein Refseq: NP_000596
• MIM: 147562
• UniProt ID: P01563