Active Recombinant Human HDAC11 Protein

• Cat.No.: HDAC11-315H
• Product Overview: Recombinant Human HDAC11 Protein is produced by HEK293 cells expression system.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Protein Length: amino acids 1–347
• Description: HDAC11 is a 39kDa protein and belongs to the Histone Deacetylase Class IV subfamily. The enzyme is primarily localized in the nucleus and shows high expression in specific tissues like brain, heart, skeletal muscle and kidney. It is the newest member of the HDAC family and hence very little is known about its functional role. Initial studies identified HDAC11 as a negative regulator of IL-10 production in APC cells. A recent study shows that HDAC11 is the most efficient fatty acid deacetylase of the HDAC family with catalytic efficiencies towards dodecanoylated and myristoylated substrates exceeding 70,000 M-1 s -1. Increasing body of evidence indicate a possible involvement of HDAC11 in diverse pathophysiological processes including immunomodulation, cancer pathogenesis, cellular differentiation etc.
• Form: Liquid, In 30 mM HEPES, 140 mM NaCl, 10 mM KCl, 3% glycerol, 0.25 mM TCEP, pH 7.6.
• Bio-activity: Deacetylation activity was determined using a myristoylated substrate. Reactions were carried out in a 384-well plate in 20 µL of reaction buffer comprising of 50 mM HEPES, 140 mM NaCl, 10 mM KCl, 1 mg/ml bovine serum albumin (BSA), and 1 mM TCEP, pH 7.4, for 30 min at 37°C. The reaction was stopped by addition of 20 µl of trypsin solution (2 mg/ml trypsin) and the fluorescence signal of released AMC was quantified using a fluorometer. The enzyme kinetic values were calculated by nonlinear regression analysis.
• Molecular Mass: 84 kDa
• Purity: ≥80% by SDS-PAGE
• Notes: For Research Use Only! Not For Use in Humans.
• Storage: Store at -80 centigrade.
• Handling: Thaw on ice, and aliquot into smaller working quantities to avoid multiple freeze/thaw cycles.

Gene Information

• Gene Name: HDAC11 histone deacetylase 11 [ Homo sapiens ]
• Official Symbol: HDAC11
• Synonyms: HDAC11; histone deacetylase 11; HD11; FLJ22237
• Gene ID: 79885
• mRNA Refseq: NM_001136041
• Protein Refseq: NP_001129513
• MIM: 607226
• UniProt ID: Q96DB2