Active Recombinant Human HDAC10, GST-tagged

• Cat.No.: HDAC10-1354H
• Product Overview: Recombinant human HDAC10 (1-482) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 1-482 aa
• Description: HDAC10 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. HDAC10 belongs to class II of the histone deacetylase family that catalyzes the deacetylation of lysine residues in the N-terminal tail of histones and represses transcription in large multiprotein complexes with transcriptional co-repressors. Therefore, HDAC10 plays a role in transcriptional repression. HDAC10 interacts with HDAC3 in co-transfected embryonic kidney cells. Deletion analysis indicates that both the N- and C-terminal domains of HDAC10 bound HDAC3 independently.
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 120 RLU/min/ng
• Molecular Mass: ~77 kDa
• Purity: >80%
• Applications: HDAC Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: HDAC10 histone deacetylase 10 [ Homo sapiens ]
• Official Symbol: HDAC10
• Synonyms: HDAC10; histone deacetylase 10; DKFZP761B039; HD10; MGC149722; DKFZp761B039
• Gene ID: 83933
• mRNA Refseq: NM_001159286
• Protein Refseq: NP_001152758
• MIM: 608544
• UniProt ID: Q969S8
• Chromosome Location: 22q13.31
• Pathway: NOTCH1 Intracellular Domain Regulates Transcription, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by NOTCH, organism-specific biosystem; Signaling by NOTCH1, organism-specific biosystem; Signaling events mediated by HDAC Class I, organism-specific biosystem; Signaling events mediated by HDAC Class II, organism-specific biosystem
• Function: NAD-dependent histone deacetylase activity (H3-K14 specific); NAD-dependent histone deacetylase activity (H3-K9 specific); NAD-dependent histone deacetylase activity (H4-K16 specific); enzyme binding; histone deacetylase activity; histone deacetylase activity (H3-K16 specific); histone deacetylase binding; hydrolase activity; protein binding; protein deacetylase activity