Active Recombinant Human HAVCR2 Protein, His-Avi-tagged, Biotinylated

• Cat.No.: HAVCR2-705H
• Product Overview: Biotinylated Human TIM-3 / HAVCR2, His Tag is expressed from HEK293 cells. It contains AA Ser 22 - Arg 200 (Accession # Q8TDQ0-1). This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 22-200 a.a.
• Predicted N Terminal: Ser 22
• Form: Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Immobilized Anti-TIM3 MAb, Human IgG4 at 2 ug/mL (100 uL/well) can bind Biotinylated Human TIM-3, His Tag with a linear range of 0.4-13 ug/mL.
• Molecular Mass: The protein has a calculated MW of 23.1 kDa. The protein migrates as 45-55 kDa under reducing condition (SDS-PAGE) due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: HAVCR2 hepatitis A virus cellular receptor 2 [ Homo sapiens ]
• Official Symbol: HAVCR2
• Synonyms: HAVCR2; hepatitis A virus cellular receptor 2; FLJ14428; Tim 3; TIM3; TIMD3; kidney injury molecule-3; T-cell membrane protein 3; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; KIM-3; Tim-3; TIMD-3; HAVcr-2
• Gene ID: 84868
• mRNA Refseq: NM_032782
• Protein Refseq: NP_116171
• MIM: 606652
• UniProt ID: Q8TDQ0