Active Recombinant Human Growth Differentiation Factor 1

• Cat.No.: GDF1-717H
• Product Overview: Recombinant Human GDF1protein was expressed in CHO. Asp254-Arg372.

Specification

• Species: Human
• Source: CHO
• Tag: Non
• ProteinLength: 254-372 a.a.
• Description: GDF1 is a 12-15 kDasecreted protein in the TGFβ superfamily. It is the functional equivalent offrog, zebrafish, and chick Vg1. Human GDF1 is synthesized as a preproproteinthat contains a 29 amino acid (aa) signal peptide, a 224 aa propeptide, and a119 aa mature peptide which is released following proteolysis at a dibasic cleavagesite. Mature human GDF1 shares 81% aa sequence identity with mouse and ratGDF1. GDF1 is expressed in the developing and adult nervous system,particularly in neurons of the cerebral cortex and hippocampus.
• Form: Lyophilized from a0.2 μm filtered solution in HCl with BSA as a carrier protein.
• N-terminalSequence: Asp254
• Molecular Weight: 12.6 kDa
• Activity: Measuredby its ability to induce Smad2 phosphorylation in P19 mouse embryonalcarcinoma cells. Mazerbourg, S. et al. (2004) Mol. Endocrinol. 18:653. 50ng/mL of Recombinant Human GDF1 can effectively induce Smad2 phosphorylation.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 13 kDa,reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100μg/mL in 4 mM HCl that containing at least 0.1% human or bovine serum albumin.
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.

Gene Information

• Gene Name: GDF1 growth differentiationfactor 1 [ Homo sapiens ]
• Official Symbol: GDF1
• Synonyms: GDF1; growthdifferentiation factor 1; DORV; DTGA3; embryonic growth/differentiationfactor 1; GDF-1
• Gene ID: 2657
• mRNA Refseq: NM_001492
• Protein Refseq: NP_001483
• MIM: 602880
• UniProt ID: P27539
• Chromosome Location: 19p13.11
• Pathway: Developmental Biology; Signaling by NODAL
• Function: cytokine activity; growth factor activity