Active Recombinant Human GREM1 protein, Fc-tagged

• Cat.No.: GREM1-1496H
• Product Overview: Recombinant Human GREM1 protein(Lys25-Asp184), fused with Fc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 25-184 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with 1 mM EDTA.
• Bio-activity: Immobilized Human BMP-2, Tag Free at 2 μg/mL (100 μL/well) can bind Human Gremlin, Fc Tag with a linear range of 0.2-8 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 44.2 kDa. The protein migrates as 48-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Lys25-Asp184
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: GREM1 gremlin 1 [ Homo sapiens ]
• Official Symbol: GREM1
• Synonyms: GREM1; gremlin 1; CKTSF1B1, cysteine knot superfamily 1, BMP antagonist 1 , gremlin 1, cysteine knot superfamily, homolog (Xenopus laevis); gremlin-1; DAND2; DRM; gremlin; gremlin 1-like protein; DAN domain family member 2; increased in high glucose-2; proliferation-inducing gene 2; increased in high glucose protein 2; cell proliferation-inducing gene 2 protein; cysteine knot superfamily 1, BMP antagonist 1; gremlin 1, cysteine knot superfamily, homolog; down-regulated in Mos-transformed cells protein; PIG2; IHG-2; GREMLIN; CKTSF1B1; MGC126660
• Gene ID: 26585
• mRNA Refseq: NM_001191322
• Protein Refseq: NP_001178251
• MIM: 603054
• UniProt ID: O60565