Active Recombinant Human GPT2 protein, His-tagged

• Cat.No.: GPT2-23H
• Product Overview: Recombinant human GPT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: Alanine aminotransferase 2, also known as GPT2, catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Subsequently, they play a key role in the intermediary metabolism of glucose and amino acids. GPT2 expression is high in muscle, fat and kidney.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH7.5) containing 30% glycerol, 2mM DTT, 0.2M NaCl
• Bio-activity: Specific activity is >100units/mg, and is defined as theamount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minuteat pH 7.5 at 37C
• Molecular Mass: 60.3 kDa (546aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMQRAAAL VRRGCGPRTP SSWGRSQSSA AAEASAVLKV RPERSRRERI LTLESMNPQV KAVEYAVRGPIVLKAGEIEL ELQRGIKKPF TEVIRANIGD AQAMGQQPIT FLRQVMALCT YPNLLDSPSF PEDAKKRARR ILQACGGNSL GSYSASQGVN CIREDVAAYI TRRDGGVPAD PDNIYLTTGA SDGISTILKI LVSGGGKSRT GVMIPIPQYP LYSAVISELD AIQVNYYLDE ENCWALNVNELRRAVQEAKD HCDPKVLCII NPGNPTGQVQ SRKCIEDVIH FAWEEKLFLL ADEVYQDNVY SPDCRFHSFK KVLYEMGPEY SSNVELASFH STSKGYMGEC GYRGGYMEVI NLHPEIKGQL VKLLSVRLCP PVSGQAAMDI VVNPPVAGEE SFEQFSREKE SVLGNLAKKA KLTEDLFNQVPGIHCNPLQG AMYAFPRIFI PAKAVEAAQA HQMAPDMFYC MKLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPVEKLKTV LQKVKDFHIN FLEKYA
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: GPT2 glutamic pyruvate transaminase (alanine aminotransferase) 2 [ Homo sapiens ]
• Official Symbol: GPT2
• Synonyms: GPT2; glutamic pyruvate transaminase (alanine aminotransferase) 2; alanine aminotransferase 2; ALT2; GPT 2; glutamic--alanine transaminase 2; glutamic--pyruvic transaminase 2; glutamic-pyruvate transaminase 2; glutamate pyruvate transaminase 2
• Gene ID: 84706
• mRNA Refseq: NM_001142466
• Protein Refseq: NP_001135938
• MIM: 138210
• UniProt ID: Q8TD30
• Chromosome Location: 16q12.1
• Pathway: Alanine, aspartate and glutamate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, conserved biosystem; Amino acid synthesis and interconversion (transamination), organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; alanine biosynthesis II, conserved biosystem
• Function: L-alanine:2-oxoglutarate aminotransferase activity; L-alanine:2-oxoglutarate aminotransferase activity; pyridoxal phosphate binding; transaminase activity