Active Recombinant Human GPC5 protein, His-tagged

• Cat.No.: GPC5-3332H
• Product Overview: Recombinant Human GPC5 protein(NP_004457.1)(Met1-Thr554), fused with His tag, was expressed in Insect Cells.

Specification

• Source: Insect Cells
• Species: Human
• Tag: His
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human GPC5 at 5 μg/ml (100 μl/well) can bind human bFGF with a linear range of 0.156-2.5 ng/ml.
• Molecular Mass: The secreted recombinant human GPC5 consists of 540 amino acids and predicts a molecular mass of 60.5 kDa as estimated in SDS-PAGE under reducing conditions.
• Protein length: Met1-Thr554
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: GPC5 glypican 5 [ Homo sapiens ]
• Official Symbol: GPC5
• Synonyms: GPC5; glypican 5; glypican-5; glypican proteoglycan 5; bA93M14.1
• Gene ID: 2262
• mRNA Refseq: NM_004466
• Protein Refseq: NP_004457
• MIM: 602446
• UniProt ID: P78333