Active Recombinant Human GPC3 Protein (Gln 25 - His 559), Fc-tagged, FITC-Labeled

• Cat.No.: GPC3-338H
• Product Overview: Recombinant Human GPC3 Protein, Fc-tagged, FITC-Labeled is expressed from human HEK293 cells. It contains AA Gln 25 - His 559 (Accession # P51654). It is the FITC labeled form of Human Glypican 3, Fc Tag. Predicted N-terminus: Gln 25. This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 87.4 kDa. The protein migrates as 43 kDa, 58 kDa and 100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. Conjugate (FITC): Excitation source: 488 nm spectral line, argon-ion laser; Excitation Wavelength: 488 nm; Emission Wavelength: 535 nm. The FITC to protein molar ratio is 3-5.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Anti-Human GPC3 MAb at 10 μg/mL (100 μL/well) can bind Recombinant Human GPC3 Protein, Fc-tagged, FITC-Labeled with a linear range of 0.078-1.25 μg/mL (Ex.488 nm/Em.535 nm) (QC tested).
• Molecular Mass: 87.4 kDa
• Protein length: Gln 25 - His 559
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: GPC3 glypican 3 [ Homo sapiens ]
• Official Symbol: GPC3
• Synonyms: GPC3; glypican 3; SDYS; glypican-3; DGSX; OCI 5; SGB; SGBS; SGBS1; MXR7; OCI-5; GTR2-2
• Gene ID: 2719
• mRNA Refseq: NM_001164617
• Protein Refseq: NP_001158089
• MIM: 300037
• UniProt ID: P51654