Active Recombinant Human GPC3, Fc-tagged

• Cat.No.: GPC3-620H
• Product Overview: The recombinant human GPC3 extracellular or ecto-domain (ECD) is expressed as a 762 amino acid protein consisting of Gln25-Val558 region of GPC3 (Uniprot accession# P51654-3) and a C-terminal Fc fusion from human IgG1, which exists as multiple proteolytically processed species under reducing conditions.

Specification

• Source: Human cells
• Species: Human
• Tag: Fc
• Form: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free).
• Bio-activity: Binds human FGF-basic/FGF2 in a functional ELISA.
• Molecular Mass: Calculated molecular mass (kDa): 86.2; Estimated by SDS-PAGE under reducing condition (kDa): 3 major bands at 40, 60 and 95-125 kDa.
• Endotoxin: <0.1 eu per 1 μg of purified recombinant protein determined by the lal>
• Purity: >90% judged by SDS-PAGE under reducing condition
• Storage: The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks. Avoid repeated freeze-thaw cycles.
• Protein length: Gln25-Val558

Gene Information

• Gene Name: GPC3 glypican 3 [ Homo sapiens ]
• Official Symbol: GPC3
• Synonyms: GPC3; glypican 3; SDYS; glypican-3; DGSX; glypican proteoglycan 3; OCI 5; SGB; SGBS; SGBS1; secreted glypican-3; intestinal protein OCI-5; heparan sulphate proteoglycan; MXR7; OCI-5; GTR2-2
• Gene ID: 2719
• mRNA Refseq: NM_001164617
• Protein Refseq: NP_001158089
• MIM: 300037
• UniProt ID: P51654
• Chromosome Location: Xq26
• Pathway: Glypican 3 network, organism-specific biosystem; Glypican pathway, organism-specific biosystem
• Function: heparan sulfate proteoglycan binding; peptidyl-dipeptidase inhibitor activity; protein binding

Reviews

08/12/2020

The GPC3 protein offered by the manufacturer is of exceptional quality, making it an ideal choice to fulfill my experimental needs.

09/18/2019

The technical support provided by the manufacturer is invaluable in overcoming experimental hurdles and optimizing the utilization of GPC3 protein.

04/10/2019

Another crucial aspect where the manufacturer excels is their efficient supply chain management.