Active Recombinant Human GLP1R protein, His-Avi-tagged, Biotinylated

• Cat.No.: GLP1R-1485H
• Product Overview: Biotinylated Recombinant Human GLP1R protein(Ala21-Glu139), fused with His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 21-139 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Biotinylated Human GLP1R, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Anti-GLP-R1 Antibody with a linear range of 0.5-31 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)The protein has a calculated MW of 17.3 kDa. The protein migrates as 30-34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ala21-Glu139
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: GLP1R glucagon-like peptide 1 receptor [ Homo sapiens ]
• Official Symbol: GLP1R
• Synonyms: GLP1R; glucagon-like peptide 1 receptor; GLP-1R; GLP-1-R; GLP-1 receptor; MGC138331
• Gene ID: 2740
• mRNA Refseq: NM_002062
• Protein Refseq: NP_002053
• MIM: 138032
• UniProt ID: P43220