Active Recombinant Human GCK Protein, His-tagged

• Cat.No.: GCK-005H
• Product Overview: Recombinant human Hexokinase4, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase4 is found in the liver and pancreatic beta-cells, where it is controlled by insulin (activation) and glucagon (inhibition). In pancreatic beta-cells, type IV enzyme acts as a glucose sensor to modify insulin secretion. Hexokinase4 is commonly called ‘glucokinase’.
• Form: Liquid
• Bio-activity: Specific activity is > 2,000 pmol/min/μg. One unit will convert 1 pmoles of D-Glucose to D-Glucose-6-phosphate per minute at pH8.0 at 37 centigrade.
• Molecular Mass: 54.3 kDa (485aa)
• AA Sequence: MGSSHHHHHHSSGLVPRGSHMLDDRARMEAAKKEKVEQILAEFQLQEEDLKKVMRRMQKEMDRGLRLETHEEASVKMLPTYVRSTPEGSEVGDFLSLDLGGTNFRVMLVKVGEGEEGQWSVKTKHQMYSIPEDAMTGTAEMLFDYISECISDFLDKHQMKHKKLPLGFTFSFPVRHEDIDKGILLNWTKGFKASGAEGNNVVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDHQCEVGMIVGTGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGDSGELDEFLLEYDRLVDESSANPGQQLYEKLIGGKYMGELVRLVLLRLVDENLLFHGEASEQLRTRGAFETRFVSQVESDTGDRKQIYNILSTLGLRPSTTDCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPSCEITFIESEEGSGRGAALVSAVACKKACMLGQ
• Endotoxin: < 1.0 EU per 1μg of protein (determined by LAL method)
• Purity: > 95% by SDS-PAGE
• Notes: For research use only. This product is not intended or approved for human, diagnostics or veterin.
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 or -70 centigrade.
  Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/mL (determined by Bradford assay)
• Storage Buffer: In 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Gene Information

• Gene Name: GCK glucokinase [ Homo sapiens (human) ]
• Official Symbol: GCK
• Synonyms: Hexokinase type IV; Glucokinase; GCK; GK; GLK; HK4; HHF3; HKIV; HXKP; LGLK; MODY2; FGQTL3; glucokinase; ATP:D-hexose 6-phosphotransferase; HK IV; glucokinase (hexokinase 4); hexokinase D, pancreatic isozyme; hexokinase type IV; EC 2.7.1.2
• Gene ID: 2645
• mRNA Refseq: NM_000162
• Protein Refseq: NP_000153
• MIM: 138079
• UniProt ID: P35557