Active Recombinant Human GAST protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: GAST-1487H
• Product Overview: Biotinylated Recombinant Human GAST protein, fused with Avi and Fc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc&Avi
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Anti-Gastrin-17 antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human Gastrin-17, Fc,Avitag with a linear range of 0.1-2.5 ng/mL.
• Molecular Mass: Molecular CharacterizationThis protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™)The protein has a calculated MW of 30.3 kDa. The protein migrates as 34-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: GAST gastrin [ Homo sapiens ]
• Official Symbol: GAST
• Synonyms: GAST; gastrin; GAS
• Gene ID: 2520
• mRNA Refseq: NM_000805
• Protein Refseq: NP_000796
• MIM: 137250
• UniProt ID: P01350