Active Recombinant Human GAS6, His-tagged

• Cat.No.: GAS6-623H
• Product Overview: Recombinant Human GAS6 (Accession # NP_000811) Ala49-Ala678, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Ala49
• Form: Lyophilized from a 0.2 µm filtered solution in Tris, NaCl and Citrate.
• Bio-activity: Measured in a cell proliferation assay using T98G human glioblastoma cells.The ED50 for this effect is typically 100-400 ng/mL. Measured by its binding ability in a functional ELISA.When Recombinant Human Axl Fc Chimera is immobilized at 2 µg/mL (100 µL/well), the concentration of Recombinant Human Gas6 that produces 50% of the optimal binding response is approximately 2.5-15 ng/mL.
• Molecular Mass: Recombinant Human GAS6, His-tagged has a calculated MW of 70.5 kDa. In SDS-PAGE migrates as 70-85 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Protein length: 49-678 a.a.

Gene Information

• Gene Name: ITGA6 integrin, alpha 6 [ Homo sapiens ]
• Official Symbol: ITGA6
• Synonyms: ITGA6; integrin, alpha 6; integrin alpha-6; CD49f; integrin alpha6B; CD49 antigen-like family member F; VLA-6; ITGA6B; FLJ18737; DKFZp686J01244
• Gene ID: 3655
• mRNA Refseq: NM_000210
• Protein Refseq: NP_000201
• MIM: 147556
• UniProt ID: P23229
• Chromosome Location: 2q31.1