Active Recombinant Human GALNS, HIS-tagged

• Cat.No.: GALNS-111H
• Product Overview: Human GALNS recombinant protein, Ala27-His522, with an N-terminal 6-His tag, was expressed in baculovirus expression system.

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: His
• Protein Length: 27-522 a.a.
• Description: This gene encodes N-acetylgalactosamine-6-sulfatase which is a lysosomal exohydrolase required for the degradation of the glycosaminoglycans, keratan sulfate, and chondroitin 6-sulfate. Sequence alterations including point, missense and nonsense mutations, as well as those that affect splicing, result in a deficiency of this enzyme. Deficiencies of this enzyme lead to Morquio A syndrome, a lysosomal storage disorder.
• Predicted N Terminal: His & Thr56
• Form: Supplied as a 0.2 µm filtered solution in Tris and NaCl. See Certificate of Analysis for details.
• Bio-activity: Measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS).
• Molecular Mass: Predicted Molecular Mass:56 kDaSDS-PAGE:53-59 kDa, reducing conditions
• Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles.6 months from date of receipt, -20 to -70 °C as supplied.3 months, -20 to -70 °C under sterile conditions after opening.

Gene Information

• Gene Name: GALNS galactosamine (N-acetyl)-6-sulfatase [ Homo sapiens (human) ]
• Official Symbol: GALNS
• Synonyms: GAS; MPS4A; GalN6S; GALNAC6S; N-acetylgalactosamine-6-sulfatase; chondroitinase; galNAc6S sulfatase; chondroitinsulfatase; galactose-6-sulfate sulfatase; N-acetylgalactosamine-6-sulfate sulfatase; galactosamine (N-acetyl)-6-sulfate sulfatase
• Gene ID: 2588
• mRNA Refseq: NM_000512
• Protein Refseq: NP_000503
• MIM: 612222
• UniProt ID: P34059
• Chromosome Location: 16q24.3
• Pathway: Chondroitin sulfate degradation, organism-specific biosystem; Chondroitin sulfate degradation, conserved biosystem; Glycosaminoglycan degradation, conserved biosystem
• Function: N-acetylgalactosamine-4-sulfatase activity; N-acetylgalactosamine-6-sulfatase activity; sulfuric ester hydrolase activity