Active Recombinant Human G Protein-Coupled Receptor Associated Sorting Protein 1, His-tagged

• Cat.No.: GPRASP1-5633H
• Product Overview: RecombinantHuman GPRASP1 protein was expressed in Murine myeloma cellline. Leu35-His576,with a C-terminal 6-His tag.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 35-576 a.a.
• Description: GPRASP1 was isolated in a screen to identify proteins in micethat copurify with myostatin (GDF-8), a potent negative regulator of skeletalmuscle mass. The GPRASP1 cDNA encodes a 571amino acid protein that contains a 29 amino acid signal sequence and iscomprised of many conserved domains: WAP, follistatin/Kazal, immunoglobulin,two tandem Kunitz, and netrin. Two related human proteins which contain thesame domain structure are called WFIKKN (based on the presence and order ofthese conserved domains) and WFIKKNRP (WFIKKN-related protein).
• Form: Lyophilized from a0.2 um filtered solution in PBS with BSA as a carrier protein.
• N-terminalSequence: Leu35
• MolecularWeight: 60.7 kDa
• Activity: Measuredby its ability to inhibit rmGDF-8 activity in K562 human chronic myelogenousleukemia cells. The ED50 for this effect is typically 0.03-0.15 μg/mL, in thepresence of 40 ng/mL of rmGDF-8. Measured by its ability to inhibit trypsin cleavageof a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp)-NH2. The IC50 valueis approximately 1.5 nM, as measured in a reaction mixture containing 1.0 nMtrypsin,10 mM ES002, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, pH 7.5.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 75-80kDa, reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 10 μg/mLin sterile PBS containing at least 0.1% human or bovine serum albumin
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 monthsfrom date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C understerile conditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.

Gene Information

• Gene Name: GPRASP1 G protein-coupledreceptor associated sorting protein 1 [ Homo sapiens ]
• Official Symbol: GPRASP1
• Synonyms: GPRASP1; Gprotein-coupled receptor associated sorting protein 1; GASP;GASP1; GASP-1; G-protein coupled receptor-associated sorting protein 1; KIAA0443
• Gene ID: 9737
• mRNA Refseq: NM_001184727
• Protein Refseq: NP_001171656
• MIM: 300417
• UniProt ID: Q5JY77
• Chromosome Location: Xq22.1
• Function: protein binding