Active Recombinant Human Fucosyltransferase 8 (alpha (1, 6) fucosyltransferase), His-tagged

• Cat.No.: FUT8-577H
• Product Overview: Recombinanthuman FUT8 protein was expressed in Chinese Hamster Ovary cell line, CHO-derived;with an N-terminus human CD33 signal sequence, and 6-His tag. Asp32-Lys575.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: 32-575 a.a.
• Description: FUT8 is frequentlyfound at terminal sites on various glycols and is essential for thegeneration of many sugar epitomes. Well known FUT8 containing glycols includeLewis and ABO blood group antigens. Lewis epitomes are key elements involvedin leukocyte homing and extravasations process, thus are essential forlymphocyte maturation and natural defense functions.
• Form: Supplied as a 0.2 μmfiltered solution in Tris and NaCl.
• N-terminal Sequence: His
• Molecular Weight: 64 kDa
• Activity: Measured by itsability to hydrolyze the donor substrate GDP-fucose. The specific activity is> 0.75 pmole/min/μg, as measured under the described conditions.
• Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
• SDS-PAGE: 58-62 kDa, reducingconditions
• Purity: >90%, by SDS-PAGEunder reducing conditions and visualized by Colloidal Coomassie Blue stain at5 μg per lane.
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3months, -20 to -70 °C under sterile conditions after reconstitution.

Gene Information

• Gene Name: FUT8 fucosyltransferase 8 (alpha(1, 6) fucosyltransferase) [ Homo sapiens ]
• Official Symbol: FUT8
• Synonyms: FUT8; fucosyltransferase8 (alpha (1, 6) fucosyltransferase); alpha-(1, 6)-fucosyltransferase;OTTHUMP00000245300; OTTHUMP00000245301; OTTHUMP00000245302; OTTHUMP00000245303;OTTHUMP00000245304; OTTHUMP00000245305; OTTHUMP00000245306; OTTHUMP00000245307;OTTHUMP00000183477; OTTHUMP00000183478; OTTHUMP00000245299; EC 2.4.1.68; Fucosyltransferase8; GDP-L-Fuc: N-acetyl-beta-D-glucosaminide alpha 1, 6-fucosyltransferase;GDP-fucose-glycoprotein fucosyltransferase; Glycoprotein; 6-alpha-L-fucosyltransferase;alpha1-6FucT; MGC26465
• Gene ID: 2530
• mRNA Refseq: NM_004480
• Protein Refseq: NP_004471
• MIM: 602589
• UniProt ID: Q9BYC5
• Chromosome Location: 14q24.3
• Pathway: AsparagineN-linked glycosylation; Glycosaminoglycan biosynthesis-keratan sulfate;Metabolic pathways; Metabolism of proteins; N-Glycanbiosynthesis; N-glycan antennae elongation in the medial/trans-Golgi;N-glycan biosynthesis, complex type; N-glycan biosynthesis, complex type;Reactions specific to the complex N-glycan synthesis pathway; Transcriptionalmisregulation in cancer; Transport to the Golgi and subsequent modification
• Function: SH3domain binding; glycoprotein 6-alpha-L-fucosyltransferase activity;transferase activity, transferring glycosyl groups