Active Recombinant Human FRK, GST-tagged

• Cat.No.: FRK-1408H
• Product Overview: Recombinant human FRK (208-end) was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.

Specification

• Description: FRK (fyn-related kinase) or Rak is a nuclear tyrosine kinaseand member of the Src sub-family. Restricted expression of FRK is detected in a broad range of cell lines with highest levels in epithelial cells. Increased expression of FRK has been shown in breast and renal cell carcinoma cell lines. In addition the retinoblastoma tumor susceptibility gene product pRb associates with FRK in vitro and in vivo. Overexpression of FRK in beta-cells from the pancreas increases the susceptibility of these cells to beta-cell-toxic events (hallmark of Type I diabetes)(2).
• Source: Sf9 Cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 997 nmol/min/mg
• Molecular Mass: ~60 kDa
• Protein length: 208 aa-end
• Purity: >90%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: FRK fyn-related kinase [ Homo sapiens ]
• Official Symbol: FRK
• Synonyms: FRK; fyn-related kinase; PTK5, PTK5 protein tyrosine kinase 5; tyrosine-protein kinase FRK; GTK; RAK; protein-tyrosine kinase 5; PTK5 protein tyrosine kinase 5; nuclear tyrosine protein kinase RAK; PTK5
• Gene ID: 2444
• mRNA Refseq: NM_002031
• Protein Refseq: NP_002022
• MIM: 606573
• UniProt ID: P42685
• Chromosome Location: 6q21-q22.3
• Function: ATP binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; protein binding