Active Recombinant Human FOLR1 protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: FOLR1-1466H
• Product Overview: Biotinylated Recombinant Human FOLR1 protein(Arg25-Met233), fused with Avi and Fc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: 25-233 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.
• Bio-activity: Immobilized Folic acid-BSA conjugate at 5 μg/mL (100 μL/well) can bind Biotinylated Human FOLR1, Fc,Avitag with a linear range of 0.2-5 ng/mL.Immobilized Biotinylated Human FOLR1, Fc,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5μg/well) plate can bind Anti-FOLR1 Antibody,Mouse IgG1 with a linear range of 0.1-2 ng/mL.Immobilized Anti-FOLR1 Antibody,Mouse IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human FOLR1, Fc,Avitag with a linear range of 0.2-5 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).The protein has a calculated MW of 53.3 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Arg25-Met233
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: FOLR1 folate receptor 1 (adult) [ Homo sapiens ]
• Official Symbol: FOLR1
• Synonyms: FOLR1; folate receptor 1 (adult); FOLR; folate receptor alpha; FR-alpha; KB cells FBP; folate binding protein; folate receptor, adult; adult folate-binding protein; ovarian tumor-associated antigen MOv18; FBP
• Gene ID: 2348
• mRNA Refseq: NM_000802
• Protein Refseq: NP_000793
• MIM: 136430
• UniProt ID: P15328