Active Recombinant Human FLT3 protein, GST/His-tagged

• Cat.No.: FLT3-4902H
• Product Overview: Human FLT3, C-terminal fragment, amino acids R571-S993 (as in NCBI/Protein entry NP_004110.2), internal tandem duplication(oncological mutation: ITD) of aa 591-600 N-terminal GST-HIS6 fusion protein with a 3C cleavage site, was expressed in Sf9 insect cells

Specification

• Description: This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
• Source: Insect cells
• Species: Human
• Tag: His&GST
• Form: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol
• Bio-activity: 15 pmol/µg×min
• Molecular Mass: 78,552 Da
• AA Sequence: RYESQLQMVQ VTGSSDNEYF YVDFREYEYD LKWEFPRENL EFGKVLGSGA FGKVMNATAY GISKTGVSIQ VAVKMLKEKA DSSEREALMS ELKMMTQLGS HENIVNLLGA CTLSGPIYLI FEYCCYGDLL NYLRSKREKF HRTWTEIFKE HNFSFYPTFQ SHPNSSMPGS REVQIHPDSD QISGLHGNSF HSEDEIEYEN QKRLEEEEDL NVLTFEDLLC FAYQVAKGME FLEFKSCVHR DLAARNVLVT HGKVVKICDF GLARDIMSDS NYVVRGNARL PVKWMAPESL FEGIYTIKSD VWSYGILLWE IFSLGVNPYP GIPVDANFYK LIQNGFKMDQ PFYATEEIYI IMQSCWAFDS RKRPSFPNLT SFLGCQLADA EEAMYQNVDG RVSECPHTYQ NRRPFSREMD LGLLSPQAQV EDS
• Storage: Storage temperature: -80 centigradeFor complete recovery, mix well and spin before use. Product must not be stored in diluted solutions, aliquots below 10µl are not advisable. Avoid repeated freeze-thaw cycles!
• Concentration: 0.095 µg/µl
• Protein length: 571-993;591-600 a.a.

Gene Information

• Gene Name: FLT3 fms-related tyrosine kinase 3 [ Homo sapiens ]
• Official Symbol: FLT3
• Synonyms: FLT3; fms-related tyrosine kinase 3; receptor-type tyrosine-protein kinase FLT3; CD135; FLK2; STK1; STK-1; CD135 antigen; FL cytokine receptor; fetal liver kinase 2; fms-like tyrosine kinase 3; stem cell tyrosine kinase 1; growth factor receptor tyrosine kinase type III; FLK-2
• Gene ID: 2322
• mRNA Refseq: NM_004119
• Protein Refseq: NP_004110
• MIM: 136351
• UniProt ID: P36888
• Chromosome Location: 13q12
• Pathway: Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Pathways in cancer, organism-specific biosystem
• Function: ATP binding; cytokine receptor activity; nucleotide binding; phosphatidylinositol 3-kinase binding; protein homodimerization activity; receptor activity; transmembrane receptor protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; vascular endothelial growth factor-activated receptor activity