Active Recombinant Human FGL1 protein, Fc-tagged

• Cat.No.: FGL1-1453H
• Product Overview: Recombinant Human FGL1 protein(Leu23-Ile312), fused with Fc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 23-312 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5.
• Bio-activity: Immobilized Human LAG-3, Mouse IgG2a Fc Tag at 10 μg/mL (100 μL/well) can bind Human FGL1, Fc Tag with a linear range of 0.02-0.156 μg/mL.Serial dilutions of Anti-LAG3, Neutralizing Antibody were added into Human FGL1, Fc Tag : Biotinylated Human LAG-3, Mouse IgG2a Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.42303 μg/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the N-terminus.The protein has a calculated MW of 60.5 kDa. The protein migrates as 60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Leu23-Ile312
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: FGL1 fibrinogen-like 1 [ Homo sapiens ]
• Official Symbol: FGL1
• Synonyms: FGL1; fibrinogen-like 1; fibrinogen-like protein 1; HFREP 1; hepassocin; liver fibrinogen-related protein 1; hepatocellular carcinoma-related sequence; hepatocyte-derived fibrinogen-related protein 1; HFREP1; HP-041; LFIRE1; LFIRE-1; MGC12455
• Gene ID: 2267
• mRNA Refseq: NM_004467
• Protein Refseq: NP_004458
• MIM: 605776
• UniProt ID: Q08830