Active Recombinant Human FGFR2 protein, His-tagged

• Cat.No.: FGFR2-621H
• Product Overview: Recombinant Human FGFR2 protein(NP_000132.3)(Met1-Glu377), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Glu377
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit FGF acidic dependent proliferation of Balb/c3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.2-1 μg/mL.
• Molecular Mass: The recombinant human FGFR2 consists of 367 amino acids after removal of the signal peptide and has a calculated molecular mass of 41 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhFGFR2 is approximately 65-75 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: FGFR2 fibroblast growth factor receptor 2 [ Homo sapiens ]
• Official Symbol: FGFR2
• Synonyms: FGFR2; fibroblast growth factor receptor 2; bacteria expressed kinase , BEK, CFD1, craniofacial dysostosis 1 , Jackson Weiss syndrome , JWS, keratinocyte growth factor receptor , KGFR; CD332; CEK3; Crouzon syndrome; ECT1; K SAM; Pfeiffer syndrome; TK14; TK25; FGFR-2; FGF receptor; soluble FGFR4 variant 4; bacteria-expressed kinase; hydroxyaryl-protein kinase; keratinocyte growth factor receptor; BEK fibroblast growth factor receptor; protein tyrosine kinase, receptor like 14; BEK; JWS; CFD1; KGFR; BFR-1; K-SAM; FLJ98662
• Gene ID: 2263
• mRNA Refseq: NM_000141
• Protein Refseq: NP_000132
• MIM: 176943
• UniProt ID: P21802