Active Recombinant Human FGFR2, GST-tagged

• Cat.No.: FGFR2-86H
• Product Overview: Recombinant human FGFR2 (K641R) (285-end) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag.

Specification

• Description: The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in this gene are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis. Multiple alternatively spliced transcript variants encoding different isoforms have been noted for this gene.?
• Source: Sf9 insect cells using baculovirus
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: The specific activity of FGFR2 (K641R) was determined to be 110 nmol/min/mg as per activity assay protocol.
• Molecular Mass: ~72 kDa
• AA Sequence: 285-end (K641R)
• Purity: The purity of FGFR2 (K641R) was determined to be >90% by densitometry.
• Applications: Kinase Assay
• Stability: 1 yr at –70oC from date of shipment.
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 ug/ul
• Protein length: 285-end a.a.

Gene Information

• Gene Name: FGFR2?fibroblast growth factor receptor 2 [?Homo sapiens(human) ]
• Official Symbol: FGFR2
• Synonyms: FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM; fibroblast growth factor receptor 2; fibroblast growth factor receptor 2; FGFR-2; FGF receptor; soluble FGFR4 variant 4; bacteria-expressed kinase; hydroxyaryl-protein kinase; keratinocyte growth factor receptor; BEK fibroblast growth factor receptor; protein tyrosine kinase, receptor like 14; EC 2.7.10.1
• Gene ID: 2263
• mRNA Refseq: NM_000141
• Protein Refseq: ?NP_000132
• MIM: 176943
• UniProt ID: P21802
• Chromosome Location: 10q26
• Pathway: Activated point mutants of FGFR2; Adaptive Immune System; Angiogenesis
• Function: ATP binding; fibroblast growth factor binding; fibroblast growth factor-activated receptor activity