Active Recombinant Human FGF6 Protein (169 aa)

• Cat.No.: FGF6-412F
• Product Overview: Recombinant human Fibroblast Growth Factor-6 (rhFGF-6) produced in E. coli is a single non-glycosylated polypeptide chain containing 169 amino acids. A fully biologically active molecule, rhFGF-6 has a molecular mass of 18.8 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: E.coli
• Protein Length: 169
• Description: Fibroblast Growth Factor-6 (FGF-6) is a cytokine belonging to the heparin-binding FGF family, and is structurally related to other members of FGF family, particularly FGF-4. In vivo, FGF-6 exhibits an expression profile predominantly restricted tothe myogenic lineage, and it preferentially binds to two of the FGF receptors: FGFR1 and FGFR4. FGF-6 functions in muscle regeneration, myoblast proliferation and migration, and muscle differentiation in a dose-dependent manner. In vivo high concentration of recombinant FGF-6 up-regulates and down-regulates FGFR1 and FGFR4, respectively, as FGFR1 promotes the proliferation while FGFR4 promotes the differentiation in the muscle. Besides its dual function in muscle regeneration, FGF-6 may act as a regulator of bone metabolism as well.
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: ED50 < 2.5 ng/mL, measured by a cell proliferation assay using 3T3 cells in the presence 1 μg/mL heparin, corresponding to a specific activity of > 4 × 10^5 units/mg.
• Molecular Mass: 18.8 kDa, observed by reducing SDS-PAGE.
• AA Sequence: MGTRANNTLLDSRGWGTLLSRSRAGLAGEIAGVNWESGYLVGIKRQRRLYCNVGIGFHLQVLPDGRISGTHEENPYSLLEISTVERGVVSLFGVRSALFVAMNSKGRLYATPSFQEECKFRETLLPNNYNAYESDLYQGTYIALSKYGRVKRGSKVSPIMTVTHFLPRI
• Endotoxin: < 0.2 EU/μg, determined by LAL method.
• Purity: > 95% as analyzed by SDS-PAGE and HPLC.
• Storage: Lyophilized recombinant human Fibroblast Growth Factor-6 (rhFGF-6) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rhFGF-6 remains stable up to 2 weeks at 4 centigrade or up to 3 months at -20 centigrade.
• Storage Buffer: Lyophilized after extensive dialysis against PBS.
• Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Gene Information

• Gene Name: FGF6 fibroblast growth factor 6 [ Homo sapiens ]
• Official Symbol: FGF6
• Synonyms: FGF6; fibroblast growth factor 6; FGF-6; HST-2; HSTF-2; heparin-binding growth factor 6; heparin secretory-transforming protein 2; HST2; HBGF-6
• Gene ID: 2251
• mRNA Refseq: NM_020996
• Protein Refseq: NP_066276
• MIM: 134921
• UniProt ID: P10767