Active Recombinant Human FGF23 Protein (Tyr25-Ile251), C-His tagged, Animal-free, Carrier-free

• Cat.No.: FGF23-298H
• Product Overview: Recombinant Human FGF23 Protein (Tyr25-Ile251) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Fibroblast Growth Factors-23 (FGF-23) is a 28 kDa member of the fibroblast Growth Factors with 251 amino acid residues. FGF-23 is expressed from brain, hepatic stellate cells, cone photoreceptor cells, early spermatids. FGF-23 involved phosphate metabolism and vitamin D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce proliferation in BaF3 cells transfected with human FGFRIIIc. The ED50 for this effect is < 0.3 μg/mL.
• Molecular Mass: The protein has a calculated MW of 26.27 kDa.
  The protein migrates as 26 kDa under reducing condition (SDS-PAGE analysis).
• Protein length: Tyr25-Ile251
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: FGF23 fibroblast growth factor 23 [ Homo sapiens (human) ]
• Official Symbol: FGF23
• Synonyms: FGF23; fibroblast growth factor 23; ADHR; FGFN; HYPF; HFTC2; HPDR2; PHPTC; fibroblast growth factor 23; phosphatonin; tumor-derived hypophosphatemia inducing factor
• Gene ID: 8074
• mRNA Refseq: NM_020638
• Protein Refseq: NP_065689
• MIM: 605380
• UniProt ID: Q9GZV9